Figure 6.

GateID Allows Enrichment of α and β Cells from Human Pancreatic Islets

(A) t-SNE map of the complete pancreas dataset (all pancreas TDs and unstained enrichment experiment datasets, n = 10,176 cells). Single cells are colored based on cell type.

(B) Left panel: PCA of human pancreas TD2 (unstained, BD FACSJazz). Each point represents a single cell, and single cells are colored based on cell type identification from scRNA-seq. The ellipses represent normal contour lines that contain 50% of the data points for each cell type. Right panel: PC1 and PC2 loadings. Each point represents a FACS channel measured by the BD FACSJazz.

(C) Contour plots of unstained human pancreas cells showing experimental gates used to sort α cells from donor 4. Sorted cells passed through gate 1 and gate 2. Percentages of events within each gate are indicated.

(D) t-SNE map of human pancreas TD2, where α cells inside and outside of GateID gates are colored red and blue, respectively.

(E) Contour plots of unstained human pancreas cells showing experimental gates used to sort β cells from donor 3. Sorted cells passed through gate 1 and gate 2. Percentages of events within each gate are indicated.

(F) t-SNE map of human pancreas TD2, where β cells inside and outside of GateID gates are colored red and blue, respectively.

(G and H) Barplots and t-SNE maps showing the outcome of GateID α and β cell enrichments for (G) donor and (H) donor 4 on BD FACSJazz. Gates were predicted on unstained TD2.

See also Figures S6 and S7 and Table S1.