(A) Study schematic design. IFN-αβR−/− mice were vaccinated IM with 109 vp of RhAd52.M-Env (RhAd52) or PBS (Sham) and challenged IV with 102 PFU ZIKV-BR 4 weeks after vaccination.
(B) Log ZIKV-specific Env binding antibody titers measured by ELISA in vaccinated versus sham mice at 4 weeks after vaccination.
(C) Log ZIKV-specific microneutralization (MN50) titers in vaccinated versus sham mice at 4 weeks after vaccination.
(D) Log ZIKV viral loads measured by RT-PCR in serum from sham mice.
(E) Log ZIKV viral loads measured by RT-PCR in serum from RhAd52 vaccinated mice.
Data is representative of one experiment with 4 animals per group. Each dot represents an individual mouse. p values were calculated using Mann-Whitney U. Mean ± SEM is shown. Related to Figure S1.