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. Author manuscript; available in PMC: 2020 Nov 13.
Published in final edited form as: Cell Host Microbe. 2019 Oct 24;26(5):591–600.e4. doi: 10.1016/j.chom.2019.10.001

Figure 3. RhAd52 and Ad26 ZIKV Vaccines Prevent Fetal Intrauterine Growth Restriction Following ZIKV Challenge.

Figure 3.

(A) Study schematic design. IFN-αβR−/− mice were vaccinated IM with 109 vp of RhAd52.M-Env (RhAd52), Ad26.M-Env (Ad26), or PBS (Sham). At week 4 after vaccination, IFN-αβR−/− dams were crossed with wildtype males. Pregnant dams were challenged IV with 102 PFU ZIKV-BR on day e5.5 followed by harvest of tissues on day e17.5. Naïve mice were not challenged.

(B) Resorption rate in Sham, RhA52, Ad26, and Naïve groups on day e17.5. Red represents resorbed fetuses, and black represents intact fetuses. The numbers of fetuses for each group are indicated on top of each bar.

(C) Representative fetuses in Sham, RhAd52, Ad26, and Naïve groups.

(D-G) Fetal body weight (grams), body length (mm), head length (mm), and CRL × OF diameter (mm2). ns (non-significant), *p = 0.021, ***p = 0.0001, ****p < 0.0001.

(H) Partial least squares discriminant analysis of biometric measurements from Sham (red), RhAd52 (black), Ad26 (blue), and Naïve (green) groups.

Data are representative of independent compiled experiments with a total of 56 to 86 animals per group. Each dot represents an individual mouse. p values were calculated using Mann-Whitney U. Mean ± SEM is shown. Related to Figure S2.