(A-C) IFN-αβR−/− mice were vaccinated IM with 109 vp of RhAd52.M-Env (RhAd52), Ad26.M-Env (Ad26), or PBS (Sham). At week 4 after vaccination, IFN-αβR−/− dams were crossed with wildtype males. Pregnant dams were challenged IV with 102 PFU ZIKV-BR on day e5.5 followed by harvest of tissues on day e17.5. Naïve mice were not challenged.
(A) Log ZIKV viral loads measured by RT-PCR in fetal resorptions.
(B) Log ZIKV viral loads measured by RT-PCR in placenta.
(C) Log ZIKV viral loads measured by RT-PCR in fetal brain.
Data are representative of independent experiments with 56 to 86 animals per group. For vaccinated groups data are plotted for individual dams (M1–M9 (RhAd52) and M1–M10 (Ad26)). Each dot represents an individual mouse. p values were calculated using Mann-Whitney U. Mean ± SEM is shown. Related to Figures S3, S4.