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. Author manuscript; available in PMC: 2020 Nov 13.
Published in final edited form as: Cell Host Microbe. 2019 Oct 24;26(5):591–600.e4. doi: 10.1016/j.chom.2019.10.001

Figure 6. Vaccine-Elicited Immune Responses Mediate Immediate Robust Protection Following ZIKV Challenge.

Figure 6.

(A) Study schematic design. Balb/c mice were vaccinated IM with 109 vp of Ad26.M-Env (Ad26) or PBS (Sham) and challenged IV with 102 PFU ZIKV-BR 4 weeks after vaccination.

(B) Log ZIKV viral loads measured by RT-PCR in serum from sham and Ad26 vaccinated mice.

(C) Log ZIKV RNA measured by RT-PCR in lymph node, spleen, ovary, and brain in sham and Ad26 vaccinated mice.

(D) Interferon-γ ELISPOT assays in response to prM, Env, Cap, and NS1 peptide pools on days 2, 4, and 7 after ZIKV challenge in sham and Ad26 vaccinated mice. Spot-forming cells per 106 splenocytes are shown.

(E) Log ZIKV-specific MN50 titers on days 0, 2, 4, and 7 after ZIKV challenge in sham and Ad26 vaccinated mice.

Data is representative of one experiment with 5 animals per group. Each dot represents an individual mouse. p values were calculated using Mann-Whitney U. Mean ± SEM is shown.