Table 1.
Primers used in this study.
| Gene name | Gene ID | Sequence 5′−3′ | % Eff | R2 | Tm (°C) | Amplicon (bp) |
|---|---|---|---|---|---|---|
| Beta actin | actb | F: TCTTCCAGCCATCCTTCCTCG R: TGTTGGCATACAGGTCCTTACGG |
92.9 | 0.999 | 60 | 108 |
| Elongation factor 1α | ef1a | F: AGAGGCTGTCCCTGGTGA R: TGATGACCTGAGCGTTGAAG |
101.6 | 0.999 | 60 | 100 |
| Glucocorticoid receptor 1 | gr1 | F: GGTTCAGCAGCAGTTCCTC R: GGTCTTGGTCGCCTTTATCC |
99.7 | 0.988 | 60 | 197 |
| Glucocorticoid receptor 2 | gr2 | F: ATCGTCAAGAGGGAGGAGAAC R: TTGGTATCTGGTTGGTGATGA |
99.5 | 0.998 | 60 | 187 |
| Mineralocorticoid receptor | mr | F: CGCCTGGCTGGAAAGCAGATG R: GAGGTCAGGGGCAAAGTAGAGCAT |
105.7 | 0.996 | 60 | 189 |
| Phosphoenolpyruvate carboxykinase | pepck | F: TCACACTCACGGACTGG R: CAATGATGGGACACTGACC |
99.8 | 0.999 | 60 | 111 |
| Fructose-bisphosphate aldolase | aldo | F: AATGTCCTTGCCAGATACGC R: TATACAGCCGCCAGAACCTT |
99.7 | 0.999 | 60 | 137 |
| Phosphoglycerate mutase 1 | pgam1 | F: AGAGGGGAAAAGGGTGCT R: CAGGCTTCAGGTTCTTGTC |
98.7 | 0.985 | 60 | 153 |
| Glucose 6 phosphatase | g6pc | F: GACCAAGAACACAAGCACCA R: AACCAGAGTCGTCGGGATTA |
97 | 0.991 | 60 | 166 |