Skip to main content
. 2019 Nov 12;10:2654. doi: 10.3389/fimmu.2019.02654

Figure 5.

Figure 5

Altered APC immunity in norovirus infected NOD mice. GF NOD mice were orally gavaged at ~4 weeks of age with pooled and filtered norovirus-enriched fecal filtrate from norovirus-infected NOD mice at 12-weeks of age that was UV-treated (virus–) or non-UV-treated (virus+). Only mice gavaged with the non-UV-treated filtrate were infected with norovirus and developed anti-MNV antibodies. (A) Cecal antibody concentrations were determined by ELISA. (B–G) The proportion of cell subsets was determined by flow cytometric analysis. (B,C) The proportion of follicular (CD21+CD23+; B) and marginal zone (CD21+CD23-; C) B cells from the pancreatic lymph nodes (PLN) and Peyer's patches (PP) were gated from live, single CD19+TCRbeta- cells prior to gating on CD21/CD23. (D,E) The proportion of naïve (IgD+IgM-) and mature (IgD+IgM+) B cells were gated as in B,C prior to gating on IgD/IgM. (F) The proportion of CXCR3+ macrophages, gated from live, single CD19-TCRbeta-IAg7(MHCII)+CD11b+CD11c- cells prior to gating on CXCR3. (G) The proportion of CD86+ macrophages gated as in F prior to gating on CD86. All data were assessed for significance using a Student's T-test. Data shown are representative of one of two experiments with n = 4 per group/experiment. *P < 0.05, **P < 0.01, ***P < 0.001.