Selumetinib effects on net cell growth of wild-type SCs and NF1 PN cells in 2D and 3D cultures. The cell populations indicated were plated with or without rBM for 24 h and then treated for 48 h using a dose-response analysis (2.5 nM, 12.8 nM, 64 nM, 320 nM, 1.6 μM, 8 μM, 40 μM) in comparison to DMSO vehicle control. Net viability was assessed using an MTT assay. Results are expressed as the mean ± SE of two replicates. 02.8-RFP = ipn02.8-RFP, 97.4-RFP = ipn97.4-RFP, 95.11b C-RFP = ipNF95.11b C-RFP, 95.6-RFP = ipNF95.6-RFP.