Figure 2.

Biofilm formation of Acinetobacter baumannii at various growth conditions. (A) The measures of adherence to microtiter plates (OD₅₇₀ nm) during growth at 26°C, 30°C, and 37°C in nutrient-rich medium (TSB) without shaking were significantly different (p<0.05; Tukey’s test) among the three temperatures tested, indicating that the variance in their means cannot be by chance but by the temperature. (B) Growth at the same nutrient-rich medium (TSB) with shaking only caused significant variation in the means (p=0.05) between groups 26°C and 37°C. (C) Biofilm-forming ability within the groups (26°C and 37°C) and (30°C and 37°C), respectively, were significantly different (p<0.05; Tukey’s comparison test) but not among the three groups when cultured in minimal medium (EAOB) with shaking. (D) There is no significant difference (p>0.05) among the three groups when cultured in EAOB without shaking, indicating that biofilm growth was optimized at this condition.