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. 2019 Nov 15;12:3523–3536. doi: 10.2147/IDR.S203919

Table 1.

Biofilm Formation of Acinetobacter baumannii Isolates (n=71) Following Incubation at 26°C, 30°C and 31°C Under Dynamic or Static Conditions in Nutrient-Rich (TSB) or Nutrient-Poor (EAOB) Media, Respectively

Physicochemical factor Non-Adherent Weak Adherent Moderate Adherent Strong Adherent Total
N (%) Average N (%) Average N (%) Average N (%) Average N (%) Average
OD ± SD OD ± SD OD ± SD OD ± SD OD ± SD
26°C EAOB dynamic 0 0 8 (11.3) 0.09 ± 0.01 38 (53.5) 0.17 ± 0.02 25 (35.2) 0.25 ± 0.02 71 (100) 0.19±0.02
26°C EAOB static 0 0 1 (1.4) 0.09 ± 0.01 31 (43.7) 0.18 ± 0.02 39 (54.92) 0.25 ± 0.01 71 (100) 0.22 ±0.01
26°C TSB dynamic 0 0 0 0 65 (91.5) 0.25 ± 0.01 6 (8.5) 0.30 ± 0.01 71 (100) 0.26 ±0.01
26°C TSB static 0 0 0 0 48 (67.6) 0.27 ± 0.01 23 (32.4) 0.29 ± 0.01 71 (100) 0.28 ±0.01
30°C EAOB dynamic 0 0 10 (14.1) 0.10 ± 0.01 53 (74.6) 0.19 ± 0.02 8 (11.3) 0.28 ± 0.02 71 (100) 0.18±0.02
30°C EAOB static 3 (4.23) 0.11 ± 0.01 28 (39.4) 0.18 ± 0.02 40 (56.3) 0.25 ± 0.01 71 (100) 0.22 ±0.02
30°C TSB dynamic 0 0 2 (2.8) 0.10 ± 0.02 69 (97.2) 0.25 ± 0.02 0 0 71 (100) 0.24 ±0.02
30°C TSB static 0 0 26 (36.6) 0.22 ± 0.02 45 (63.4) 0.26 ± 0.01 0 0 71 (100) 0.24 ±0.01
37°C EAOB static 3 (4.2) 0.10 ± 0.00 26 (36.6) 0.16 ± 0.01 42 (59.27) 0.25 ± 0.02 0 0 71 (100) 0.21 ±0.01
37°C EAOB static 0 0 11 (15.5) 0.15 ± 0.01 54 (76.1) 0.23 ± 0.01 4 (5.6) 0.30 ± 0.01 71 (100) 0.21 ±0.02
37°C TSB dynamic 2 (2.8) 0.10 ± 0.02 23 (32.4) 0.20 ± 0.01 46 (64.8) 0.26 ± 0.01 0 0 71 (100) 0.23 ±0.02
37°C TSB static 3 (4.2) 0.10 ± 0.01 56 (78.9) 0.21 ± 0.02 12 (16.9) 0.28 ± 0.01 0 0 71 (100) 0.22 ±0.02

Notes: Biofilm formation assay data is the mean of three independent experiments carried out in triplicate SD after growth in minimal (EAOB) and rich (TSB) media at 26°C, 30°C, and 37°C under dynamic and static conditions, respectively.