Table 2.
Primers and molecular beacon for the Salmonella spp. real-time nucleic acid sequence–based amplification assay
| Target organism | Gene | Primer beacon | Sequence(5′-3′) | Reference |
|---|---|---|---|---|
| Salmonella spp. | xcd | Pxcd-f | 5′-GTTAGCTGGTATCTGGATGA-3′ | This study |
| Pxcd-f | 5′-AATTCTAATACGACTCACTATAGGG1 AAACTGATGGTTATAAGCATAGGT-3′ | This study | ||
| Bxcd | FAM-CGATCG2CGTATACCGGT AACCAGGAGGGGACGATCG2-DABCYL | This study | ||
| invA | 139 | GTGAAATTATCGCCACGTTCGGGCAA | [16] | |
| 141 | TCATCGCACCGTCAAAGGAACC | [16] |
1: Recognition sequence of T7 RNA polymerase
2: Reverse repetitive sequence