Organization of the hydrolase/tRNACys region from P. larvae (upper panel) and tRNACys-PCR products (lower panel). Primers were designed to amplify the hydrolase-tRNACys region in P. larvae. Lane 1, primer combination Pan1-Pan2 (1268 bp product), lane 2, Pan3-Pan4 (907 bp product), lane 3, Pan1-Pan4 (1218 bp product), and lane 4, Pan2-Pan3 (957 bp product). Lane M contains a Thermo Scientific GeneRuler 100 bp DNA ladder