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Images of confocal laser microscopy. Biofilm produced during 46 h by the mixed culture of S. aureus COL, S. aureus FRI 722, and S. aureus Embrapa 4018 on polystyrene surface in the absence of nisin. The green fluorescence represents live cells stained with FITC while red and yellow show dead cells stained with propidium iodide. Image generating biofilm slices with standardized cuts of 1.0 μm
