. 2019 Jun 11;50(4):1099–1103. doi: 10.1007/s42770-019-00101-6

Table 2.

Phenotypic and genetic features of atypical enteropathogenic (aEPEC) and Shiga toxin-producing (STEC) Escherichia coli isolates obtained from ground beef and poultry breast purchased in Botucatu, São Paulo, Brazil

Origin	Food sample	E. coli isolates	DEC pathotype^a	Serotype^b	DEC virulence markers				Additional virulence factor-encoding genes^d	HeLa cells		Stx^g	Phylo-group
Origin	Food sample	E. coli isolates	DEC pathotype^a	Serotype^b	eae (intimin subtype)^c	bfpB	stx₁	stx₂	Additional virulence factor-encoding genes^d	Adherence pattern^e	FAS^f	Stx^g	Phylo-group
Ground beef	11	11–1	aEPEC	O105:H7	+ (θ)	–	–	–	nleB, nleE	UND	+	NT	B1
	18	18–5	aEPEC	O4:H16	+ (β1)	–	–	–	–	LAL	+	NT	A
	20	20–1	STEC	ONT:H46	–	–	–	+	iha, saa, ehxA, espP	UND	–	+	B1
Poultry breast	28	28–3	aEPEC	O68:H12	+ (β1)	–	–	–	–	UND	+	NT	A
	45	45–3	aEPEC	OR:H25	+ (NT)	–	–	–	–	UND	+	NT	A
	47	47–1	aEPEC	O8:H25	+ (NT)	–	–	–	–	UND	+	NT	A
	47	47–3	aEPEC	O8:H25	+ (NT)	–	–	–	–	UND	+	NT	A

^aDEC: diarrheagenic E. coli; aEPEC: atypical enteropathogenic E. coli; STEC: Shiga toxin-producing E. coli

^bONT: somatic antigen O non-typeable with the antisera used (O1-O188); OR: rough isolates

^cIntimin subtypes: β1: beta1, θ: theta, NT: non-typeable

^dAdditional virulence factor-encoding genes investigated: iha, paa, saa, efa1/lifA, nleB, nleE, ehxA, and espP

^eAdherence patterns exhibited in HeLa cells (6 h assay) in the presence of D-mannose (2%): LAL (localized adherence-like) and UND (undefined adherence)

^fFAS, fluorescent-actin staining test

^gShiga toxin (Stx) production (Vero cells toxicity assay). NT, not tested