Table 3.
Sensitivity and specificity of LAMP-coupled LFD in comparison with fecal culture
| Assays | Specimen | No. of samples (N = 389) | Test positive |
Test negative |
False positive |
False negative |
|
|---|---|---|---|---|---|---|---|
| TP | TN | ||||||
| Culture | Fecal matter | 86 | 303 | 86 | 303 | – | – |
| PCR | DNA | 93 | 296 | 86 | 296 | 7 | – |
| LAMP LFD | DNA | 95 | 294 | 86 | 294 | 9 | – |
| Statistics | Culture | PCR | LAMP LFD | ||||
| Sensitivity | 100% | 100% | 100% | ||||
| Specificity | 100% | 97.68% | 97.02% | ||||
| Positive predictive value | 100% | 93.0% | 91.34% | ||||
| Negative predictive value | 100% | 100% | 100% | ||||
| Efficiency | 100% | 98.23% | 97.73% | ||||
*Values are represented in terms of reactivity
*Values are represented in terms of positivity; values in parenthesis represent 95% confident intervals