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. 2019 Nov 15;12:9795–9807. doi: 10.2147/OTT.S219440

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© 2019 Li et al.

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MTDH downregulation is required for the miR-618–driven inhibition of U2OS and HOS cell growth and metastasis in vitro. MiR-618–overexpressing U2OS and HOS cells were transfected with either plasmid pCMV-MTDH or the empty pCMV vector. (A) Transfected cells were collected after 72 h of incubation and subjected to Western blot analysis for the determination of MTDH protein expression. *P < 0.05 vs group miR-NC. #P < 0.05 vs the “miR-618 mimics+pCMV” group. (B–E) The proliferation, apoptosis, migration, and invasiveness of U2OS and HOS cells treated as described above were investigated by the CCK-8 assay, flow-cytometric analysis, and Transwell migration and invasion assays, respectively. *P < 0.05 vs group miR-NC. #P < 0.05 vs the miR-618 mimics+pCMV group.