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MiR-618 inhibits activation of the PTEN–Akt pathway in OS cells by targeting MTDH. (A) pCMV-MTDH or pCMV was transfected into U2OS and HOS cells in the presence of the miR-618 mimics. After that, protein levels of PTEN, p-AKT, and AKT were assayed by Western blotting. (B) Western blotting was utilized to determine PTEN, p-AKT, and AKT expression in U2OS and HOS cells after si-NC or si-MTDH injection.
