REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
GAPDH	Merck Millipore	MAB374, RRID:AB_2107445
LC3A/B	Sigma	L8918, RRID:AB_1079382
eIF5A	BD Biosciences	611976, RRID:AB_399397
Hypusine	Merck Millipore	ABS1064, RRID:AB_2631138
Hypusine	Creative Biolabs	PABL-202
ATG7	Abcam	Ab133528, RRID:AB_2532126
ATG3	Abcam	Ab108251, RRID:AB_10865145
H3	Cell Signaling	9715, RRID:AB_331563
S6	Cell Signaling	2317, RRID:AB_2238583
pS6 (Ser235/236)	Cell Signaling	4858, RRID:AB_916156
AMPKα	Cell Signaling	2793, RRID:AB_915794
pAMPKα (T172)	Cell Signaling	2535, RRID:AB_331250
AcK	Cell Signaling	9441, RRID:AB_331805
ATF4	Santa Cruz	sc-200, RRID:AB_2630429
eIF2α	Cell Signaling	2103, RRID:AB_836874
p-eIF2α (Ser51)	Cell Signaling	9721, RRID:AB_330951
TFEB	Bethyl	A303-673A, RID:AB_11204751
TFE3	Sigma	HPA023881
DHS	Abcam	Ab190266
β-Actin	Cell Signaling	3700, RRID:AB_2242334
CD40	Thermo Fisher	16-0401-86, RRID: AB_468943
IRDye 800CW Donkey Anti-Rabbit IgG (H+L)	LI-COR	926-32213, RRID:AB_621848
IRDye 680RD Donkey Anti-Mouse IgG (H+L)	LI-COR	926-68022, RRID:AB_10715072
IRDye 800CW Streptavidin	LI-COR	926-32230,
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568	Thermo Fisher	A-11011, RRID: AB_143157
CD16/CD32 Monoclonal Antibody (FcR Block)	Thermo Fisher	14-0161-85, RRID:AB_467134
Pacific Blue anti-mouse/human CD44 Antibody	BioLegend	103020, RRID:AB_493683
Pacific Blue anti-mouse TER-119/Erythroid Cells Antibody	BioLegend	116232, RRID:AB_2251160
Pacific Blue anti-mouse CD45.1 Antibody	BioLegend	110722, RRID:AB_492866
Pacific Blue anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody	BioLegend	108430, RRID:AB_893556
Pacific Blue anti-mouse Ly-6A/E (Sca-1) Antibody	BioLegend	122520, RRID:AB_2143237
Brilliant Violet 605 anti-mouse CD41 Antibody	BioLegend	133921, RRID:AB_2563933
Brilliant Violet 605 anti-mouse CD4 Antibody	BioLegend	100451, RRID:AB_2564591
Brilliant Violet 605 anti-mouse/human CD45R/B220 Antibody	BioLegend	103244, RRID:AB_2563312
Ly-6A/E (Sca-1) Monoclonal Antibody, PerCP-Cyanine5.5	Thermo Fisher	45-5981-82, RRID:AB_914372
CD19 Monoclonal Antibody, PerCP-Cyanine5.5	Thermo Fisher	45-0193-82, RRID:AB_1106999
FITC anti-mouse Ly-6A/E (Sca-1) Antibody	BioLegend	108106, RRID:AB_313343
FITC anti-mouse/human CD45R/B220 Antibody	BioLegend	103206, RRID: AB_312991
CD43 Monoclonal Antibody, FITC	Thermo Fisher	11-0431-81, RRID:AB_465039
FITC anti-mouse CD4 Antibody	BioLegend	100406, RRID:AB_312691
FITC anti-mouse IgM Antibody	BioLegend	406506, RRID:AB_315056
PE anti-mouse/human CD45R/B220 Antibody	BioLegend	103208, RRID:AB_312993
PE anti-mouse Ly-51 Antibody	BioLegend	108308, RRID:AB_313365
PE anti-mouse/human CD44 Antibody	BioLegend	103008, RRID:AB_312959
PE anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody	BioLegend	108408, RRID:AB_313373
PE anti-mouse CD135 Antibody	BioLegend	135306, RRID:AB_1877217
PE anti-mouse CD19 Antibody	BioLegend	115508, RRID:AB_313643
PE anti-mouse/human CD11b Antibody	BioLegend	101208, RRID:AB_312791
CD4 Monoclonal Antibody (GK1.5), PE-Cyanine5	Thermo Fisher	15-0041-82, RRID:AB_468695
PE/Cy5 anti-mouse CD5 Antibody	BioLegend	100610, RRID:AB_312739
PE/Cy5 anti-mouse CD8a Antibody	BioLegend	100710, RRID:AB_312749
PE/Cy5 anti-mouse/human CD11b Antibody	BioLegend	101210, RRID:AB_312793
PE/Cy5 anti-mouse CD11c Antibody	BioLegend	117316, RRID:AB_493566
PE/Cy5 anti-mouse/human CD45R/B220 Antibody	BioLegend	103210, RRID:AB_312995
PE/Cy5 anti-mouse CD3ε Antibody	BioLegend	100310, RRID:AB_312675
TCR beta Monoclonal Antibody, PE	Thermo Fisher	12-5961-82, RRID:AB_466066
TCR gamma/delta Monoclonal Antibody, PE-Cyanine5	Thermo Fisher	15-5711-82, RRID:AB_468804
PE/Cy5 anti-mouse TER-119/Erythroid Cells Antibody	BioLegend	116210, RRID:AB_313711
PE/Cy5 anti-mouse NK-1.1 Antibody	BioLegend	108716, RRID:AB_493590
PE/Cy5 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody	BioLegend	108410, RRID:AB_313375
PE/Cy7 anti-mouse CD43 Antibody	BioLegend	143210, RRID:AB_2564349
PE/Cy7 anti-mouse CD8a Antibody	BioLegend	100722, RRID:AB_312761
PE/Cy7 anti-mouse CD19 Antibody	BioLegend	115520, RRID:AB_313655
PE/Cy7 anti-mouse IgM Antibody	BioLegend	406514, RRID:AB_10642031
PE/Cy7 anti-mouse CD150 (SLAM) Antibody	BioLegend	115914, RRID:AB_439797
APC anti-mouse CD105 Antibody	BioLegend	120414, RRID:AB_2277914
APC anti-mouse CD3ε Antibody	BioLegend	100312, RRID:AB_312677
TCR beta Monoclonal Antibody, APC	Thermo Fisher	17-5961-81, RRID:AB_469480
TCR gamma/delta Monoclonal Antibody, APC	Thermo Fisher	17-5711-82, RRID:AB_842756
APC anti-mouse NK-1.1 Antibody	BioLegend	108710, RRID:AB_313397
APC anti-mouse/human CD45R/B220 Antibody	BioLegend	103212, RRID:AB_312997
APC anti-mouse/human CD11b Antibody	BioLegend	101212, RRID:AB_312795
APC anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody	BioLegend	108412, RRID:AB_313377
APC anti-mouse CD24 Antibody	BioLegend	138506, RRID:AB_2565651
APC anti-mouse CD4 Antibody	BioLegend	100412, RRID:AB_312697
APC anti-mouse CD48 Antibody	BioLegend	103412, RRID:AB_571997
APC anti-human CD19	BioLegend	302212, RRID:AB_314242
Alexa Fluor® 700 anti-mouse CD45.2 Antibody	BioLegend	109822, RRID:AB_493731
Alexa Fluor® 700 anti-mouse CD8a Antibody	BioLegend	100730, RRID:AB_493703
CD8a Monoclonal Antibody, APC-eFluor 780	Thermo Fisher	47-0081-82
APC/Cy7 anti-mouse/human CD11b Antibody	BioLegend	101226, RRID:AB_830642
APC/Cy7 anti-mouse IgD Antibody	BioLegend	405716, RRID:AB_10662544
APC/Cy7 anti-mouse CD19 Antibody	BioLegend	115530, RRID:AB_830707
CD117 (c-Kit) Monoclonal Antibody (2B8), APC-eFluor 780	Thermo Fisher	47-1171-82, RRID:AB_1272177
Goat Anti-Mouse IgG1, Human ads-AP	Southern Biotech	1070-04
Rabbit IgG (IP)	Santa Cruz	sc-2027, RRID:AB_737197
ATG7 (IP)	Abcam	Ab58735, RRID:AB_940290
AcK (IP)	Cell Signaling	9441, RRID:AB_331805
Biological Samples
Healthy adult PBMCs	Kennedy Institute of Rheumatology, University of Oxford	Rec: 11/h0711/7
		UoB Ref: 17-1106
Chemicals
Spermidine	Cayman Chemical	14918
Bafilomycin A1	Cayman Chemical	11038
N1-Guanyl-1,7-diaminoheptane (GC7)	Merck Millipore	259545-10MG
Difluoromethylornithine (DFMO)	Enzo Life Sciences	ALX-270-283
Cycloheximide	Sigma	C1988
4-Hydroxytamoxifen (4-OHT)	Sigma	H6278
Tamoxifen	Sigma	T5648
Torin 1	Cayman Chemical	10997
Etoposide	Cayman Chemical	12092
NP-CGG (4-Hydroxy-3-nitrophenylacetyl hapten-Chicken Gamma Globulin)	Biosearch Technologies	N-5055D
NP-BSA	Biosearch Technologies	N-5050H
Critical Commercial Assays
FlowCellect™ Autophagy LC3 Antibody-based Detection Kit	Merck Millipore	FCCH100171
Click-iT Plus OPP Protein Synthesis Assay	Thermo Fisher	C10456
Click-iT® Metabolic Labeling kits	Thermo Fisher	C10102, C10276
Deposited Data
Ribosome profiling	GEO: GSE133934
Mendeley data	https://doi.org/10.17632/cd4f7j2hbh.1
Experimental Models: Cell lines
Jurkat	ATCC	RRID:CVCL_0367
NIH 3T3	ATCC	RRID:CVCL_0594
HEK293T	ATCC	RRID:CVCL_0063
Experimental Models: Organisms/Strains
Mouse: C57BL/6J	The Jackson Laboratory	RRID:IMSR_JAX:000664
Mouse: B6.SJL	The Jackson Laboratory	RRID:IMSR_JAX:100012
Mouse: Mb1-cre	The Jackson Laboratory	RRID:IMSR_JAX:020505
Mouse: Atg7flox	(Komatsu et al., 2005)	MGI:3587769
Mouse: GFP-LC3	(Mizushima et al., 2004)	MGI:3759813
Mouse: Dhsflox	(Pällmann et al., 2015)	NA
Mouse: Tcfebflox	(Settembre et al., 2012)	NA
Mouse: CAG-cre/Esr1	The Jackson Laboratory	RRID:IMSR_JAX:004682
Oligonucleotides (siRNA)
Eif5a #1	Dharmacon	D-057410-02
Eif5a #2	Dharmacon	D-057410-04
Odc #1	Dharmacon	D-041731-17
Odc #2	Dharmacon	D-041731-03
Dhs #1	Dharmacon	D-054953-01
Dhs #2	Dharmacon	D-054953-02
Tfeb	Dharmacon	D-050607-02
Tfe3	Dharmacon	L-054750-00
Non-target	Dharmacon	D-001220-01
Recombinant DNA
pMSCV-UBC:GFP	Addgene	RRID:Addgene_68482
MISSION® 3X LacO Inducible Non-Target shRNA Control	Sigma	SHC332-1EA
TaqMan®probes
Gapdh	Thermo Fisher	Mm99999915_g1
Odc1	Thermo Fisher	Mm01964631_g1
Dohh	Thermo Fisher	Mm00505991_m1
Atg9b	Thermo Fisher	Mm01157883_g1
Ctsd	Thermo Fisher	Mm00515586_m1
Gla	Thermo Fisher	Mm00516323_m1
Neu1	Thermo Fisher	Mm00456846_m1
Psap	Thermo Fisher	Mm00478338_m1
Tfeb	Thermo Fisher	Mm00448968_m1
Wipi1	Thermo Fisher	Mm00461219_m1
GAPDH	Thermo Fisher	Hs02758991_g1
TFEB	Thermo Fisher	Hs01115721_m1
ATG7	Thermo Fisher	Hs00893770_m1
CHOP	Thermo Fisher	Hs00358796_g1
Software and Algorithms
FlowJo	FlowJo (commercial)	https://www.flowjo.com
Prism	GraphPad (commercial)	https://www.graphpad.com/scientific-software/prism/
Image Studio Lite	LI-COR (commercial)	https://www.licor.com/bio/image-studio-lite/?gclid=Cj0KCQjwpPHoBRC3ARIsALfx-_JzDOH3Ce3nVzcdgITtx4Xe0ouX62JKl-h0H5WWVPjdEPnrzl3l08waArTQEALw_wcB