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. 2019 Sep 19;179(1):219–235.e21. doi: 10.1016/j.cell.2019.08.032

Figure 2.

Figure 2

Differential Heterogeneity Induces Differential Tumor Growth In Vivo

(A) Scheme of experimental design for generating UVB-irradiated cells and generating SCCs derived from UVB-irradiated cells. Cell lines are irradiated by UVB at dosage of 600 J/m2; from these irradiated cells, SCCs are generated.

(B) Distribution of variant allele frequencies (VAFs) of parental B2905 cells (black), UVB-irradiated B2905 cells (red), SCC 1 (purple), and SCC 2 (green) in log2 space. VAF > 0.25 (log2 = −2) is considered clonal.

(C) Tumors excised from mice inoculated with either parental or UVB-irradiated cell lines on day 15 after inoculation.

(D) In vivo tumor growth in mice inoculated with parental B2905 cells (black) and UVB-irradiated cells (red). n = 3–4; data are representative of three independent experiments. Data are mean ± SEM. ∗p < 0.05, ∗∗∗p < 0.001, two-way ANOVA followed by Bonferroni’s post hoc test.

(E) Tumors excised from UVB-irradiated B2905 cells versus SCC 2, day 19.

(F) In vivo growth of tumors in mice inoculated with UVB (red) or SCC 1 (purple) and SCC 2 (green). n = 4–5; data are representative of two independent experiments. Data are mean ± SEM.

∗∗p < 0.01∗∗∗p < 0.001, two-way ANOVA followed by Bonferroni’s post hoc test. ∗ refers to UVB and SSC 1 comparisons; # refers to UVB and SSC 2 comparisons. See also Figure S2, Table S1, and Table S2.