Figure 4.

Homogeneous SCCs Elicit a Strong Immune Response

(A) Flow cytometry analysis of the Granzyme B and CD107a population in total TCRβ⁺ TILs on day 19. n = 4–5; data are mean ± SEM. ^∗∗p < 0.01 for Granzyme B⁺ CD107a⁺ TILs, two-way ANOVA followed by Bonferroni’s post hoc test.

(B) Flow cytometry analysis of interferon-γ (IFN-γ) in total TILs on day 19. n = 4–5, ^∗p < 0.05, Kruskal-Wallis test followed by Dunn’s multiple comparisons test.

(C) CYT score derived from RNA-seq data of sorted CD8⁺ TILs from UVB-irradiated B2905 and SCC 2 tumors on day 15. ^∗p < 0.05, Mann-Whitney U test.

(D) Pearson correlation between CYT score and weights of tumors in Figure 3C.

(E) Quantitation of total CD8⁺ TILs in the indicated tumors. Four sections from each tumor and three tumors derived from each cell line were examined. A significant difference was observed between parental cells and SSC 2 but not between parental cells and UVB. Data are mean ± SEM. ^∗p < 0.05, one-way ANOVA followed by Tukey’s post hoc test.

(F) Relative quantitation of the average percentage of CD8⁺ TILs in the tumor core versus the margin of the tumors described in (E). Data are mean ± SD.

(G) Representative immunohistochemical stain for CD8 in slides taken from tumors derived by parental, UVB and SCC 2 on day 10 after cell inoculation. The scale bars represent 100 μM.

(H) Immunofluorescence stains of CD3 and Foxp3 in tumors derived from B2905 parental, UVB, and SCC 2, 16, and 11 on days 10−11 after cell inoculation. 3–4 sections from each tumor and two tumors derived from each cell line were examined. The scale bars represent 200 μM.

(I) Relative quantitation of the percentage of Foxp3⁺ of CD3⁺ TILs described in (H). Data are mean ± SEM. ∗p < 0.05, one-way ANOVA followed by Tukey’s post hoc test.

See also Figure S5.