Figure 5.

Detection and Characterization of HLA-Bound Neoantigens in SCCs

(A) Left: spectra of three representative neoantigens detected by targeted mass spectrometry. The SCCs in which these neoantigens were detected are indicated. Right: surface expression of H2-D_b and H2-K_b on RMA-S cells incubated with the peptides at 0.1–100 μM for 18 h and measured by flow cytometry analysis.

(B) In vivo killing assay in mice immunized with the three neoantigens described in (A) using DC vaccination. The killing percentage is calculated relative to the killing measured in naive, non-vaccinated mice.

n = 2–3. Data are mean ± SEM. See also Figure S6 and Tables S2 and S3.