Figure S5.

Differential TIL Activation and Infiltration in Differentially Heterogeneous Tumors, Related to Figure 4

A-B) Flow cytometry results of the analyses described in Figure 4 A-B. C) Immunofluorescence of CD3 and CD8 in slides taken from tumors derived by B2905 Parental, UVB and SCC 2. Magnification is 4X. Pictures are representative of three mice per group. Scale bar represents 500 μM. D) higher magnification (10X) of the SCC 2 core. Scale bar represents 200 μM. E-F) Quantification of CD8⁺ TILs infiltration (E, ^∗p < 0.05, ^∗∗∗p < 0.001, One-way ANNOVA followed by Tuckey’s post hoc test) and their core versus margin localization in tumors derived from B2905-parental, UVB, and SCC 9,11,16, at day 15 post inoculation. Significant differences are seen between UVB and SSC 11/16 but not between UVB and parental cell lines. n = 3-4. G) Representative immunohistochemical stain for CD8⁺ cells. Slides were taken from the tumors indicated above, at day 15 post inoculation. Scale bar represents 200 μM.