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. 2019 Nov 18;51(4):476–487.e7. doi: 10.1016/j.devcel.2019.10.018

Figure 5.

Figure 5

PM Integrity and cER Curvature under Heat Stress

(A) Schematic of the propidium iodide assay to assess PM integrity (left) and PM integrity measurements of Tcb deletion mutants upon 10-min incubation at 42°C (right). The entry of propidium iodide in cells with compromised PM integrity was measured by flow cytometry. The plot shows average values (white/gray bars) for each condition ± SE (error bars). ∗, ∗∗, and ∗∗∗, respectively, indicate p < 0.05, p < 0.01, and p < 0.001 by Mann-Whitney U test (for tcb1Δ 26°C data, which was not normally distributed) or unpaired t test (for all other conditions). Four independent biological repeats were performed for all conditions. (B and C) 1.4-nm-thick tomographic slices of cER in the indicated strains (left) and 3D renderings of cER curvature (right). Agg: aggregate; cER: cortical ER; Mito: mitochondrion; Nuc: nucleus; PM: plasma membrane; Vac: vacuole. (B) WT cell under heat stress (HS). Insets show cER peaks (blue arrowhead in the tomographic slice inset). (C) tcb1/2/3Δ cell under heat stress. Scale bars: 300 nm (main panels), 25 nm (inset). See also Figures S2 and S4; Table S1.