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. 2019 Nov 18;51(4):460–475.e10. doi: 10.1016/j.devcel.2019.09.006

Figure 5.

Figure 5

Ect2 Is Recruited by Caveolae to Activate RhoA

(A) Control (left) and Ect2 knockdown (center) A2780 cells expressing membrane-targeted Raichu-RhoA in 3D CDM; right: average forward rear movement over 5 min of control, Ect2, Vav2, and PLEKHG5 knockdown cells (N>46 cells/condition, 3 repeats).

(B) Ratiometric FRET imaging of control, Ect2, Vav2, and PLEKHG5 knockdown cells; average rear RhoA FRET ratio in control, Ect2, Vav2, and PLEKHG5 knockdown cells (N>32 cells/condition, 3 repeats).

(C) Endogenous Ect2 and cavin-1 in A2780 cells in 3D-CDM; MIPs of whole cell and separately captured zoomed rear region. Right: peak Ect2 staining intensity in rear, nuclear, and front regions (N=17 cells, 3 repeats).

(D) Control (left) and caveolin-1 knockdown cells (right) in CDM stained for F-actin (phalloidin) and Ect2, MIPs shown; Rear peak/front peak Ect2 intensity levels (N>20 cells/condition, 3 repeats).

∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, not significant. See also Figure S5.