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. 2019 Oct;58:249–258. doi: 10.1016/j.sbi.2019.05.021

Figure 3.

Figure 3

CryoSTAC of HIV-1 immature Gag particles.

(a) Tomographic slices of immature HIV-1 particles (with D25A mutation), ΔMACANCSP2 VLPs, in the absence and presence of the maturation inhibitor bevirimat (BVM). Scale bar, 50 nm. (b) CA-SP1 densities from the samples shown in (a) by subtomogram averaging. One CA-SP1 monomer is highlighted, with the CA-NTD in cyan and the CA-CTD and SP1 in orange. (c) The refined atomic model. (d) An improved CA-SP1 density map at 3.1 Å resolution by emClarity (right), compared to the previous structure (left, EMD-3782). (e) Enlarged views of boxed area in (d) overlaid with a real-space refined model [18••,26••].