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. 2019 Nov 19;14(11):e0220757. doi: 10.1371/journal.pone.0220757

Fig 2. Phosphorylation of P70S6K and 4E-BP1 in response to treatment with media conditioned by ex vivo human serum (n = 6).

Fig 2

C2C12 myotubes were nutrient deprived for 1 h followed by treatment with media conditioned by ex vivo fasted (fast) or 60 min postprandial (fed) serum for 4 h. Postprandial serum was obtained 1 h after ingesting WPH or isonitrogenous NEAA. Densitometric analysis of P70S6K and 4E-BP1 phosphorylation before and after treatment with media conditioned by WPH or NEAA-fed serum (A, C) and relative to fasted serum (B, D). Representative immunoblots of phospho-P70S6K, phospho-4E-BP1, their respective total proteins and β-Actin (E). Data reported as Mean±SEM relative to their respective total proteins *within groups p<0.05, #between groups p<0.05.