Skip to main content
. 2019 Nov 7;15(11):e1008454. doi: 10.1371/journal.pgen.1008454

Fig 4. The α-Cat N domain recruits Jub to AJs.

Fig 4

(A) α-Cat protein structure. (B) Control late 3rd larval instar discs expressing en-Gal4, UAS-RFP and Jub::GFP, controlled by its endogenous promoter (upper panels), were stained for Arm (lower panels). Nuclei are labeled with DAPI. Close-up images to the right show wing pouch area on both sides of the anterior-posterior compartment boundary. Scale bars, 50 μm and 25 μm. (C) Same markers as in (A) with UAS-α-CatRNAi(1) expression in PC. (D) Late 3rd larval instar discs expressing Jub::GFP, en-Gal4, UAS-RFP, UAS-α-Cat-RNAi(1), and UAS-DEcad::αCat. (E) Late 3rd larval instar discs expressing Jub::GFP, en-Gal4, UAS-RFP, UAS-α-Cat-RNAi(1), and UAS-DEcadΔβ::αCatΔN. (F) Quantification of wing disc area of flies of indicated genotypes. Two-tailed, unpaired t-test was used to determine statistical significance; *(P = 0.0180), **(P = 0.0012). (G) Comparison of relative fluorescent intensities between AC and PC for Jub::GFP and Arm. AC values were normalized to 100%. N = 200–500 cells from three wing discs. Mann Whitney test; ****(P≤0.0001), ns (P>0.05).