(A, B) Overexpression of α-Cat causes an enlargement of the adult wing, which can be suppressed by one mutant copy of ykiB5 or jub-RNAi. Two-tailed, unpaired t-test was used to determine statistical significance. ns(P>0.05), ****(P≤0.0001). (C, D) Overexpression of α-Cat does not cause a significant enlargement of the late 3rd larval instar wing disc. However, overexpression of α-Cat in conjunction with a KD of an upstream regulator of the Hippo/Yki pathway (Fat, shown in (C), Crb and Ex shown in S3 Fig; all three interactions quantified in (D)) showed synergistic overgrowth phenotypes. Scale bars, 100 μm. Two-tailed, unpaired t-test; ****(P≤0.0001).