Figure 5:

Resveratrol treatment alters SCFA production in TNBS colitis. The study was designed as described in Fig 1 legend. SCFA were isolated from cecal contents of experimental groups through acidification using metaphosphoric acid. GC-FID analysis was performed to determine the concentrations of acetic (A), propionic (B), i-butyric (C), n-buytric (D), i-valeric (E), and n-valeric (F) acids. SCFAs were identified using standard compounds purchased from Sigma-Aldrich. Representative data from two independent experiments with 5 mice in each group is depicted. Significance (p-value: *<0.05, **<0.01, ***<0.005, ****<0.001) was determined using one-way ANOVA followed by Tukey’s post-hoc multiple comparisons test.