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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Oct 21;1866(1):165560. doi: 10.1016/j.bbadis.2019.165560

Figure 4. Regulation of ATX protein levels in human TM cells.

Figure 4.

ATX protein levels in serum starved human TM cells treated with dexamethasone (A; 0.5 μM, 96 h, daily addition), TNFα (B; 10 ng/ml, 24 h) or IL-1β (C; 10 ng/ml, 24 h) were significantly increased in both cell lysates and conditioned media compared to control cells (vehicle treated). Histograms represent the fold change in ATX protein levels under the above described conditions, based on densitometry analysis. GAPDH was used as a loading control for the cell lysates. For the conditioned media samples, equal amounts of protein samples were subjected to SDS-PAGE and one of the indicated Coomassie Brilliant blue stained protein bands was used for normalizing protein loads. * p ≤ 0.05. N = 4