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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Oct 21;1866(1):165560. doi: 10.1016/j.bbadis.2019.165560

Figure 7. Suppression of LPC induced fibrogenic activity in human TM cells by inhibition of ATX activity.

Figure 7.

Treatment of serum starved human TM cells with LPC (5 μM for 10 h) stimulated significant increase in the levels of fibronectin, collagen type I and CTGF proteins compared to control cells (A & B). These effects of LPC were significantly suppressed in cells pretreated with ATX inhibitor - PF8380 (1 μM) or ATX-A (1 μM) for 1 hr. On a relative basis, while 0.1 μM ATX-A also significantly suppressed LPC induced fibrogenic activity, 0.1 μM PF8380 exhibited only a modest effect on the ability of LPC to stimulated increase in the levels of fibronectin, collagen type 1 and CTGF. GAPDH was used as a loading control for the cell lysates. * p ≤ 0.05. N = 4