Table 1. Characteristics of A. bereziniae HPC229 plasmids.
| Plasmid | Size (kbp) | RepB (replication initiator protein) assignment a | Iteron sequences (length)b | No. of direct repeats | TA systems | MOB-subfamily classificationb | Resistance determinantsc |
|---|---|---|---|---|---|---|---|
| pAbe229-114 | 114 | pXBB1-8 (99%)/AR3G4 | 5´-AAGTGGAAGGCCTGTCACTAAT-3´ (22 bp)* | 6 | hipA/B, fic/yhfG, higA/B, higA2/B2, hicB | MOBP111 | cadAR and cadAR-like, nrpAB, acr3, cusABCF |
| pNDM229 | 44.5 | - | - | - | zeta toxin | MOBQ1 | blaNDM-1, aphA6, bleMBL |
| pAbe229-15 | 15.3 | pMMD (100%)/AR3G3 | 5´-TAACTATGACGGATTGACTAC-3´ (21 bp)** | 4 | higA2/B2 | MOBQ1 | - |
| pAbe229-9 | 9.1 | pAV1 (85%)/AR3G6 | 5´-ACCTATACCACACCAAAAAGTC-3´ (22bp)** | 4 | splT/A, doc | MOBQ1 | - |
| pAbe229-4 | 4.4 | - | - | - | relE/B | - | - |
| pAbe229-1 | 1.3 | - | - | - | - | - | - |
aAccording to the classification of Acinetobacter Rep_3 superfamily proteins [30]. The percentages of identity to representative members of a different subgroups (AR3G) are indicated between brackets.
bIteron sequences were identified using the Tandem Repeats Finder program available at (https://tandem.bu.edu/trf/trf.html) and manually curated. An asterisk (*) indicates the presence of imperfect repeat sequences (the consensus pattern was calculated by Tandem Repeats Finder). Two asterists (**) indicate four perfect repeats.
ccadAR and cadAR-like operons encoding putative Co/Zn/Cd ions resistance systems; nrpAB operon encoding putative a Ni ion resistance system; acr3, putative arseniate resistance; cusABCF operon encoding putative Cu ion resistance system.