Table 3. Performance evaluation of PetNADTM and TRIzol RNA extraction methods for duck hepatitis A virus detection by rRT-PCR and RT-iiPCR.
| Sample ID | Sequencing result |
PetNADTM | TRIzol | ||
|---|---|---|---|---|---|
| rRT-PCR (Ct value) |
RT-iiPCR | rRT-PCR (Ct value) |
RT-iiPCR | ||
| SCGH04 | + | 27.09 | + | 26.51 | + |
| SCGH 05 | + | 30.93 | + | 28.14 | + |
| SCGH08 | + | 32.11 | + | 30.07 | + |
| SCGH09 | + | 29.73 | + | 27.46 | + |
| SCGH10 | + | 29.16 | + | 29.05 | + |
| SCGH11 | + | 25.31 | + | 23.17 | + |
| SCGH12 | + | 26.44 | + | 24.32 | + |
| SCGH13 | + | 30.24 | + | 28.76 | + |
| SWUN18 | + | 31.57 | + | 25.06 | + |
| SWUN19 | + | 30.18 | + | 23.19 | + |
| SWUN20 | + | 29.36 | + | 24.18 | + |
| SWUN21 | + | 27.19 | + | 22.34 | + |
| SCMY14 | − | Neg | − | Neg | − |
| SCMY 27 | − | Neg | − | Neg | − |
| SCMY 31 | − | Neg | − | Neg | − |
| SCMY 36 | − | Neg | − | Neg | − |
| SCMY 49 | − | Neg | − | Neg | − |
| DA 14 | − | Neg | − | Neg | − |
| DY 17 | − | Neg | − | Neg | − |
| DY 32 | − | Neg | − | Neg | − |
−, negative; +, positive.