Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 16;81(10):1475–1484. doi: 10.1292/jvms.19-0057

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

©2019 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

PMC Copyright notice

Fig. 1. — Expression, purification, and characterization of the recombinant B subunit of the Escherichia coli heat-labile enterotoxin (rLTB). (A) Sodium dodecyl sulfate-Polyacrylamide gel electrophoresis (SDS-PAGE) analyses for rLTB expression at indicated time points, post initial methanol addition to culture supernatants. (B) Western blot analyses for rLTB determination. Lanes 1 and 2: purified rLTB obtained from 2 independent experiments cultured in anti-cholera toxin (CT) antibody; Lane 3: purified rLTB cultured in CT-negative serum. (C) Ganglioside M1 (GM1)-ganglioside binding assay using enzyme-linked immunosorbent assay (ELISA). GM1 ganglioside coated or non-coated plates were incubated with purified rLTB, diluted as indicated. Results are from 2 independent experiments.