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. 2019 Sep 16;81(10):1475–1484. doi: 10.1292/jvms.19-0057

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©2019 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 2. — Anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibody responses. Mice (n=6) were intranasally immunized with either saline, PRRSV antigen-only or antigen admixed with 200 µg alum, or 10, 50, or 100 µg rLTB, on days 0, 7, and 21. On day 28, sera were harvested for anti-PRRSV IgG analyses (A), and bronchoalveolar lavage (BAL) fluid (B), vaginal washing (C), and fecal pellets (D) were collected for anti-PRRSV IgA antibody measurements, using indirect ELISA. Data are expressed as mean ± SD. *P<0.05, **P<0.01, and ***P<0.001 versus the PRRSV antigen-only group.