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. 2019 Sep 16;81(10):1475–1484. doi: 10.1292/jvms.19-0057

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©2019 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 5. — Production of IFN-γ, IL-12, IL-4, IL-10, and IL-6 by splenocytes. Mice (n=6) were intranasally immunized with either saline, porcine reproductive and respiratory syndrome virus (PRRSV) antigen-only or antigen admixed with 200 µg alum, or 10, 50, or 100 µg rLTB, on days 0, 7, and 21. Splenocytes were prepared on day 28 and cultured with PRRSV antigen for 48 hr. The supernatants were tested for IFN-γ (A), IL-12 (B), IL-4 (C), IL-10 (D), and IL-6 (E) analyses, using a Luminex^® 100IS system. Data are expressed as mean ± SD. *P<0.05 and **P<0.01 versus the PRRSV antigen-only group.