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MPC-5 podocytes were randomly exposed to HG for 0, 24 and 48 h. MVs concentration was remarkably and significantly elevated (4-folds increase for 24 h, and 12-folds increase for 48 h, P<0.001). *, P<0.001, compared with control (0 h); #, P<0.001, compared with 24 h.
