(A) Time course of NOX4 mRNA expression. Podocytes were randomly exposed to HG for given time point. NOX4 mRNA expression was up-regulated immediately at 2 h to 2.6-folds of NC, and then fall back gradually to the baseline at 24 h. *, P<0.001, compared with NC (n=4). (B) Time course of NOX4 protein expression. Podocytes were randomly exposed to HG for given time point. Representative Western blot was shown, and β-tubulin was used as a loading control. NOX4 protein expression was up-regulated immediately from 2 h, and peaked at 4 h to 2.08-folds of NC, and then fall back gradually to the baseline at 24 h. *, P<0.01, compared with NC (n=4).