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. 2019 Nov 13;10:2551. doi: 10.3389/fmicb.2019.02551

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Copyright © 2019 Tseytin, Mitrovic, David, Langenfeld, Zarivach, Diepold and Sal-Man.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EscS can self-interact. Whole-cell lysates of E. coli BL21 (DE3) expressing either EscS_wt-HA or EscS_wt-V₅, were subjected to immunoprecipitation using protein G beads linked to an anti-HA antibody. The lysates were incubated alone or mixed. Whole-cell lysates and elution fractions were separated on a 16% SDS-PAGE and analyzed by western blotting with anti-HA and anti-V₅ antibodies. EscS-V₅ was co-eluted with EscS-HA.