Table 1.
Strains and plasmids used in this study.
| Strains | Description | Reference |
|---|---|---|
| Wild-type EPEC | EPEC strain E2348/69, streptomycin resistant | Iguchi et al., 2009 |
| EPEC ΔescS | Non-polar deletion of escS | Tseytin et al., 2018b |
| EPEC ΔescN | Non-polar deletion of escN | Gauthier et al., 2003 |
| EPEC ΔescD | Non-polar deletion of escD | Tseytin et al., 2018a |
| Y. enterocolitica MRS40 | Clinical isolate containing wild-type pYV plasmid E40 (pYVe40) ΔblaA | Sory et al., 1995 |
| Y. enterocolitica AD4016 | pYVmrs40 egfp-yscQ | Diepold et al., 2010 |
| Y. enterocolitica AD4034 | pYVmrs40 egfp-yscQ ΔyscS | Diepold et al., 2010 |
| Y. enterocolitica AD4173 | pYVmrs40 yscV-egfp | Diepold et al., 2011 |
| Y. enterocolitica AD4179 | pYVmrs40 yscV-egfp ΔyscS | Diepold et al., 2011 |
| E. coli DH10B | For plasmid handling | Durfee et al., 2008 |
| E. coli FHK12 | An E. coli strain in which the ctx promoter was fused to a lacZ gene | Kolmar et al., 1995 |
| E. coli PD28 | a malE-deficient E. coli strain | Duplay et al., 1987 |
| E. coli BL21 (λDE3) | For protein expression | Promega |
| E. coli Top10 | For plasmid handling | Thermo Fisher |
| PLASMIDS | ||
| pEscSwt-HA (pSA10) | HA C-terminal tagged EscS in pSA10 | This study |
| pEscSwt-V5 [pET28a(+)] | V5 C-terminal tagged EscS in pET28a(+) | This study |
| pEscR-3HA (pSA10) | 3HA C-terminally tagged EscR in pSA10 | Tseytin et al., 2018b |
| pEscT-2HA (pSA10) | 2HA C-terminally tagged EscT in pSA10 | This study |
| pEscV-His (pSA10) | Penta-his C-terminally tagged EscV in pSA10 | This study |
| pEscUN262A-HA (pTOPO) | 2HA C-terminally tagged EscU in pTOPO – uncleavable version | Zarivach et al., 2008 |
| pEscS-TMD1ex-HA (pSA10) | C-terminal tagged EscS with an 7L9A sequence instead of the original TMD1 in pSA10 | This study |
| pEscS-TMD2ex-HA (pSA10) | C-terminal tagged EscS with an 7L9A sequence instead of the original TMD2 in pSA10 | This study |
| pEscSK54A-HA (pSA10) | C-terminal tagged EscS with a point mutation at position 54 | This study |
| pEscS-TMD1ex-V5 [pET28a(+)] | C-terminal tagged EscS with an 7L9A sequence instead of the original TMD1 in pET28a(+) | This study |
| pEscS-TMD2ex-V5 [pET28a(+)] | C-terminal tagged EscS with an 7L9A sequence instead of the original TMD2 in pET28a(+) | This study |
| pEscD-V5 (pACYC184) | V5 C-terminally tagged EscD in pACYC184 | Tseytin et al., 2018a |
| pAD654 | pBAD::YscS | This study |
| pAD655 | pBAD:: YscS(P23A) | This study |
| pAD656 | pBAD:: YscS (K54A) | This study |
| pAD657 | pBAD:: YscS-FLAG | This study |
| pAD658 | pBAD:: YscS (K54A)-FLAG | This study |
| pAD659 | pBAD:: YscS (P23A)-FLAG | This study |
| ToxR-GpA-MBP | The GpA TMD sequence inserted between ToxR and MBP | Langosch et al., 1996 |
| ToxR-A16-MBP | A sequence of 16 alanine residues inserted between ToxR and MBP | Langosch et al., 1996 |
| ToxR-7L9A-MBP | A sequence of 7 leucine residues and 9 alanine residues inserted between ToxR and MBP | Sal-Man et al., 2005 |
| pToxR-TMD1-MBP | The first TMD sequence of EscS inserted between ToxR and MBP | This study |
| pToxR-TMD2-MBP | The second TMD sequence of EscS inserted between ToxR and MBP | This study |
| pET28a(+) | Expression vector for His-tagging, Kanr | Novagen |