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. 2019 Nov 13;13:1191. doi: 10.3389/fnins.2019.01191

Figure 2.

Figure 2

Dynamic expression of EphA4 in a rat sciatic nerve crush model. (A) Representative Western blot images showing EphA4, MAG, Krox-20, and MPZ levels in regenerating nerve segment at indicated different time points (i.e., 1, 4, 7, 14, 21, and 28 days) following nerve crush, and normal nerve was used as the control. Histogram compared the protein expression change of EphA4, MAG, Krox20, and P0 in regenerating nerve segment at indicated different time points following nerve crush. GAPDH served as a loading control. * or & or # or αp < 0.05, ** or && or ## or ααp < 0.01, ###p < 0.001 vs. control, one-way ANOVA, n = 3 rats/group. The symbols, as *, &, # and α, represent the EphA4, Krox-20, MAG and P0, respectively. (B) Immunofluorescence labeling for S100β (red) and EphA4 (green) in the injured sciatic nerve sections of rats showing the colocalization of EphA4 and S100β from day 7 of injury, indicating that EphA4 was expressed by Schwann cells from day 7 after nerve injury. Scale bar, 500 μm. Boxed insets are high magnification pictures, Scale bar, 200 μm. The representative immunohistochemical images were selected from three independent experiments.