Figure 3.

Dynamic expression of EphA4 in DRG neurons co-culture with Schwann cells. (A) Immunocytochemistry with anti-MAG (green) and anti-MPZ (red) displayed the myelinated axons. Also shown are the higher magnifications of the boxed areas. Scale bar, 100 μm. Western blotting was used to detect the expression of MAG and MPZ during myelination. Primary culture SCs were used as the control (n = 3, t-test, ^**p < 0.01). (B) The gene expression changes of EphA4, Krox-20, MPZ, and MAG in the process of myelination (n = 3, one-way ANOVA, ^*p < 0.05, ^**p < 0.01 vs. SCs).