Figure 6.

EphA4 knockdown accelerated Schwann cell differentiation. (Aa1) Immunocytochemistry with anti-MAG (green) displayed the SC differentiation efficacy of the different inducers that were cultured in vehicle or with dB CAMP treatment. Scale bar, 100 μm. (Aa2) Western blotting compared the MAG expression in differentiated cells treated with dB CAMP for 48 and 72 h, and vehicle as control. Also shown (Insert) are representative western blotting images, in which GAPDH served as an internal standard. ^*p < 0.05, ^***p < 0.001, vs. vehicle, t-test, n = 3 cultures per group. (Aa3–a5) The gene expression changes of EphA4 (a3), Krox-20 (a4) and MAG (a5) during SC differentiation (n = 3, t-test, ^**p < 0.01 vs. control). (B) Western blot showing EphA4 and MAG in differentiated SCs following knockdown of EphA4 in SCs. Histograms showing that EphA4 knockdown increase the expression of MAG compared with transfection with negative control (n = 3, t-test, ^**p < 0.01).