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. 2019 Nov 13;10:2568. doi: 10.3389/fmicb.2019.02568

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Copyright © 2019 Hashimoto, Taniguchi, Uesaka, Nomura, Hirakawa, Tanimoto, Tamai, Ruan, Zheng and Tomita.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PFGE of S1 nuclease-treated or -untreated DNA of the AA708 strain in different running conditions. (A) The pulse time was from 1.0 to 23 s during 18.5 h of electrophoresis. Lanes: Lambda Ladder PFG Marker (NEB); S1 nuclease-untreated DNA; S1 nuclease-treated DNA. (B) The pulse time was from 50 to 90 s during 22 h of electrophoresis. Lanes: Lambda Ladder PFG Marker (NEB); S1 nuclease-untreated DNA; S1 nuclease-treated DNA. (C) The pulse time was from 1.0 to 12 s during 15 h of electrophoresis. Lanes: Lambda Ladder PFG Marker (NEB); S1 nuclease-untreated DNA; S1 nuclease-treated DNA.