FIGURE 8.

Retardation assay of pELF1 DNAs excised from PFGE or SDS-PFGE gels. DNA bands of pELF1 were excised from PFGE gel (proteinase K treatment +) or SDS-PFGE gel (proteinase K treatment –), and digested with SacI (lanes 3 and 4), and SmaI (lanes 5 and 6). After digestion, the samples were subjected to PFGE. MM, Low Range PFG Marker; 1, pELF1 excised from PFGE gel; 2, pELF1 excised from SDS-PFGE gel; 3, SacI-treated pELF1 excised from PFGE gel; 4, SacI-treated pELF1 excised from SDS-PFGE gel; 5, SmaI-treated pELF1 excised from PFGE gel; 6, SmaI-treated pELF1 excised from SDS-PFGE gel. N. D.; not digested.