TABLE 1.
Gene | Length (bp) | Primers | Sequence | References |
hflX | 128 | hflX-F | 5′-TGTAGGTGAAGGTAAAGCAG-3′ | This study |
hflX-R | 5′-CACGACACTCGCACAAACGC-3′ | |||
fliA | 112 | fliA-F | 5′-GCTGGCTGTTATTGGTGTCG-3′ | Chen et al., 2018 |
fliA-R | 5′-CAACTGGAGCAGGAACTTGG-3′ | |||
motA | 120 | motA-F | 5′-CTTCCTCGGTTGTCGTCTGT-3′ | Chen et al., 2018 |
motA-R | 5′-CTATCGCCGTTGAGTTTGGT-3′ | |||
ompA | 152 | ompA-F | 5′-TCCAGAGCAGCCTGACCTTC-3′ | This study |
ompA-R | 5′-GCTGAGCCTGGGTGTTTCCT-3′ | |||
gapA | 104 | gapA-F | 5′-GAAATGGGACGAAGTTGGTG-3′ | Congrui et al., 2013 |
gapA-R | 5′-AACCACTTTCTTCGCACCAG-3′ | |||
luxS | 116 | luxS-F | 5′-TGCCACACTGGTAGACGTTC-3′ | Chen et al., 2018 |
luxS-R | 5′-TGATTGGTACGCCAGATGAG-3′ | |||
pfS | 169 | pfs-F | 5′-CGGCAACAGCCAGGAACTCA-3′ | This study |
pfs-R | 5′-GCGAAAATCCGCCACAACTT-3′ | |||
sdiA | 105 | sdiA-F | 5′-AGTCTGATGGCTCTGATGCG-3′ | This study |
sidA-R | 5′-CTTACCTTCCGCCGTCCATT-3′ | |||
ftsE | 81 | ftsE-F | 5′-AAAGTACCCTCCTGAAGCTGATCTGTG-3′ | This study |
ftsE-R | 5′-GCGTGATGTCATGGCCGCTAAAC-3′ | |||
ftsQ | 95 | ftsQ-F | 5′-GTTTCTTCTCGCCGCAATAAT-3′ | This study |
ftsQ-R | 5′-AACACGACCCAGCCGCTCACC-3′ |
The primers for the target genes were designed using the NCBI primer designing tool.