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. 2019 Nov 13;9:386. doi: 10.3389/fcimb.2019.00386

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Copyright © 2019 Vargas-Montes, Cardona, Moncada, Molina, Zhang and Gómez-Marín.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection limit of RH antigen according time and temperature. The ELAA assays were performed with AP001 (A,C) and AP002 (B,D) anti-ROP18 aptamers. We evaluated incubation 1 h at 37°C (A,B) and 4°C overnight (C,D). Different concentrations of T. gondii total antigen of the RH strain (RH Ag) were evaluated and three negative controls, PDI, LucGT, and BSA were included. The data are represented with the mean of each sample evaluated in triplicate. Welch's t-test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 vs. controls without antigen (No Ag).