Skip to main content
. 2019 Nov 13;9:386. doi: 10.3389/fcimb.2019.00386

Figure 3.

Figure 3

Evaluation of different blocking conditions and streptavidin dilutions for AP001 (A,C) and AP002 (B,D) ELAA test. Different blocking conditions were evaluated: 1% bovine serum albumin, 5% skim milk and no blocking (A,B). Three different dilutions of streptavidin (1: 10,000, 1: 15,000 and 1: 20,000) were evaluated (C,D). RH Ag was used as a positive control (50 μg/ml) for both experiments. rROP18 (25 μg/ml) was also used as positive control for streptavidin experiment. Three negative controls were included, PDI, LucGT, and BSA. The data are represented by the mean ± SEM. Kruskal Wallis. *p < 0.05.