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Concentration and Binding affinity of AP001 (A) and AP002 (B) aptamers. Ag RH was used as a positive control and different concentrations of each aptamer (from 50 to 500 nM) were used. BSA was used as a negative control. The dissociation constant (Kd) was calculated through a non-linear regression to define the affinity of aptamers to the RH Ag, obtaining a Kd of 62.7 nM for AP001 and a Kd of 97.7 nM for AP002 (C).
