Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 19;9:17078. doi: 10.1038/s41598-019-53225-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Activation of PKCγ is essential for neuronal differentiation of N2a. (a) Western blot analysis of PKCγ depletion in N2a cells mediated by CRISPR/Cas9. (b) Western blot analysis showed that V5-3 inhibited PKCγ activation induced by 30% OM in N2a. (c) Western blot analysis indicated various PKCγ lentiviral constructions were expressed in N2a. (d) PKCγ depletion inhibited neuronal differentiation induced by 30% OM. N2a were immunostained with Tubulin III antibody and the percent of differentiated N2a cells with GFP⁺ was quantified. (e) V5-3 (20 nM) attenuated neuronal differentiation of N2a. (f) Activation of PKCγ facilitated neuronal differentiation of N2a. N2a cells were infected with indicated PKCγ constructs and induced differentiation. For d and f, the percent of differentiated cells with GFP⁺ was quantified in right panel. All data are expressed as mean ± SD (n = 3 individual experiments in each group) and compared by one-way ANOVA (a,b,d,f) or Student’s t-test (e) (**p < 0.01, ^#p < 0.05 vs indicated group). Scale bars, 20 μm.